Journal: Arthritis and rheumatism

Article Title: Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.

doi: 10.1002/art.21745

Figure Lengend Snippet: Figure 1. A, Cytokine and chemokine mRNA expression in total RNA from the submandibular glands of MRL/lpr mice, during the development of sialadenitis. Total RNA (2 g) isolated from submandibular glands of 5 mice at ages 4, 8, 10, 12, 16, and 20 weeks was analyzed by reverse transcription–polymerase chain reaction (RT-PCR), as described in Materials and Methods. Representative RT-PCR results are shown. Experiments were performed in triplicate. B, Expression of interferon-inducible protein 10 (IP-10) in the submandibular glands of MRL/lpr mice, during the development of sialadenitis. Representative immunohistochemistry results are shown. Formalin-fixed sections (n 5 mice per age group) were deparaffinized, incubated with biotin-labeled rabbit anti-mouse IP-10 polyclonal antibody, and the expression of IP-10 was detected with avidin–biotin–peroxidase. The sections were counterstained with hematoxylin. IFN interferon-; IL-2R interleukin-2 receptor; Mig monokine induced by IFN; I-TAC IFN-inducible T cell chemoattractant; TARC thymus and activation–regulated chemokine; MDC monocyte-derived chemokine.

Article Snippet: Biotin-conjugated anti-mouse IP-10 goat polyclonal antibody, biotin-conjugated anti-mouse Mig goat polyclonal antibody, biotin-conjugated anti-mouse I-TAC goat polyclonal antibody, biotin-conjugated anti-mouse TARC goat polyclonal antibody, and biotin-conjugated anti-mouse MDC polyclonal antibody were obtained from R&D Systems (Minneapolis, MN).

Techniques: Expressing, Isolation, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Incubation, Labeling, Avidin-Biotin Assay, Activation Assay, Derivative Assay

Journal: Methods in Molecular Biology

Article Title: Allergy

doi: 10.1007/978-1-4939-9591-2

Figure Lengend Snippet: Fig. 1 Different types of immunoelectrophoresis of cat hair extract (Fel d) using a polyclonal rabbit antibody raised against the same extract (a Fel d). (a) CIE of Fel d extract, (b) FRIE following a purification (immunoab- sorption) of the major allergen Fel d 1, (c) CIE of purified Fel d 1 against a Fel d, (d) CLIE with Fel d 1 in inter- mediate gel for identification of the allergen, (e) TCIE with Fel d extract and Fel d 1 showing a double peak, (f) CIIE with monospecific Fel d 1 in intermediate gel

Article Snippet: Detection: Biotinylated chicken anti-human MDC polyclonal (R&D Systems #BAF336 or PeproTech).

Techniques: Immunoelectrophoresis, Purification